High-Throughput Glycomic Methods
Irena Trbojević-Akmačić, Guinevere S. M. Lageveen-Kammeijer, Bram Heijs, Tea Petrović, Helena Deriš, Manfred Wuhrer, Gordan Lauc
TL;DR
This paper reviews high-throughput methods for analyzing glycans, focusing on their role in understanding glycosylation processes in health and disease.
Contribution
The paper highlights recent advancements and challenges in high-throughput glycomics, particularly for N-glycosylation analysis.
Findings
High-throughput glycomics enables the analysis of hundreds or thousands of glycomes efficiently.
N-glycosylation is a focal point in current high-throughput glycomic profiling.
Applications include deciphering glycosylation processes in healthy and disease states.
Abstract
Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the analysis of hundreds or thousands of glycomes in a timely manner (termed high-throughput glycomics). This review provides a historic overview as well as highlights recent developments and challenges of glycomic profiling by the most prominent high-throughput glycomic approaches, with N-glycosylation analysis as the focal point. It describes the current state-of-the-art regarding levels of characterization and most widely used technologies, selected applications of high-throughput glycomics in deciphering glycosylation process in healthy and…
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Taxonomy
TopicsGlycosylation and Glycoproteins Research · Genomics and Phylogenetic Studies · Monoclonal and Polyclonal Antibodies Research