# High-Throughput Glycomic Methods

## TL;DR

This paper reviews high-throughput methods for analyzing glycans, focusing on their role in understanding glycosylation processes in health and disease.

## Contribution

The paper highlights recent advancements and challenges in high-throughput glycomics, particularly for N-glycosylation analysis.

## Key findings

- High-throughput glycomics enables the analysis of hundreds or thousands of glycomes efficiently.
- N-glycosylation is a focal point in current high-throughput glycomic profiling.
- Applications include deciphering glycosylation processes in healthy and disease states.

## Abstract

Glycomics aims to identify the structure and function
of the glycome,
the complete set of oligosaccharides (glycans), produced in a given
cell or organism, as well as to identify genes and other factors that
govern glycosylation. This challenging endeavor requires highly robust,
sensitive, and potentially automatable analytical technologies for
the analysis of hundreds or thousands of glycomes in a timely manner
(termed high-throughput glycomics). This review provides a historic
overview as well as highlights recent developments and challenges
of glycomic profiling by the most prominent high-throughput glycomic
approaches, with N-glycosylation analysis as the
focal point. It describes the current state-of-the-art regarding levels
of characterization and most widely used technologies, selected applications
of high-throughput glycomics in deciphering glycosylation process
in healthy and disease states, as well as future perspectives.