Integrate and boost: bioscaffolds nurture the cardiac regenerative paradigm

TL;DR

This paper discusses how bioscaffolds can improve heart regeneration by optimizing stem cell therapies and tissue engineering.

Contribution

The paper introduces bioscaffolds as a novel approach to enhance cardiac regenerative treatments.

Findings

Traditional stem cell therapies have shown limited clinical success due to methodological and biological challenges.

Biomaterials and tissue engineering are advancing the next generation of cardiac regenerative strategies.

Bioscaffolds offer a promising platform for improving the efficacy of heart failure treatments.

Abstract

The traditional cardiac regenerative paradigm using non-modified adult stem cells with various routes of delivery into the myocardial target has thus far yielded unconvincing clinical outcomes. Besides factors related to heterogeneity in trial methodology, inter-patient variability and the rare incidence of adult stem cells with intrinsic repair potency underscore the importance of further optimization and standardization of regenerative platforms. Cardiac tissue engineering seizing upon the advances of cellular, molecular, and biomaterial development is shaping the next generation of the regenerative paradigm and thereby fostering disruptive curative treatments in heart failure.

Full text

Introduction

In a recent article in Stem Cell Research & Therapy, Gálvez-Montón et al. [1] provide new evidence that neurovascular integration of acellular pericardial-derived scaffold may induce cardiac repair. Ischemia-induced myocyte loss is associated with degradation of the extracellular matrix and induction of replacement fibrosis as the primary trigger of adverse ventricular remodeling and heart failure progression. Cell-based regenerative interventions are under investigation as an adjunct to current standards of care in two main settings. If employed acutely after myocardial infarction, cell-based therapy is thought to facilitate myocardial recovery through a cardioprotective pathway. Conversely, in the setting of established cardiac dysfunction, the restorative impact of cell-based therapy aborts pathological remodeling and contributes to the restoration of healthy tissue. Although various clinical trials have demonstrated the safety and feasibility of these approaches, varied signals of benefits between trials have prompted careful evaluation and optimization of existing strategies.

Cardiac tissue engineering and biomaterial scaffolds offer an alternative strategy to regenerate damaged myocardium. They are applied as a patch or as an injection to overcome the hostile ischemic milieu either to facilitate the transplanted cell survival or to promote endogenous repair by facilitating electromechanical coupling and neovasculogenesis [2, 3]. This is key in ensuring the transport of nutrients and functional integration of the scaffold. Using a cell-free pericardial scaffold, the present study provides biological proof of concept for such an approach in a large animal model of acute myocardial infarction [1]. It demonstrates neovascularization and nerve formation in a cell-free pericardial bioscaffold preparation implanted early after experimental myocardial infarction. Detailed immunohistochemistry and electron microscopy analysis demonstrate the functional neovascularization without adjunctive growth factor stimulation. Neovascularization with intraluminal erythrocytes has been observed across the entire thickness of the scaffold away from the host myocardium. Furthermore, authors report de novo nerve sprouting [1]. At the ultrastructural level, these neuronal cells contain structural organelles consistent with the differentiated neuronal afferent cells. The underlying mechanism and signaling were not elucidated here. However, in contrast to synthetic scaffolds, the porous structure of prepared pericardium features preserved biological tunnels, which in the setting of hypoxia likely provide the necessary cues for the host progenitor cells facilitating their migration and scaffold integration [4]. Because the scaffold delivery here was performed early after the myocardial infarction where abundant inflammatory and reparative signals exist, it remains unclear whether a similar degree of neurovascular integration would occur in chronic myocardial infarction. In addition, the lack of a control group prevents the broader translation of infarct size reduction and effects on overall cardiac structure and function.

Aside from the inherent limitations of this biological proof-of-concept experiment, there are several implications for the framework of cardiac regeneration. The traditional cardiac regenerative paradigm using non-modified adult stem cells with various routes of delivery into the myocardial target has thus far yielded unconvincing clinical outcomes [5]. Factors related to heterogeneity in trial methodology, inter-patient variability, and the rare incidence of adult stem cells with intrinsic repair potency underscore the importance of further optimization and standardization of regenerative platforms [5]. Optimization efforts span all levels of the regenerative paradigm (Table 1). Moving beyond heterogenous biologics such as bone marrow mononuclear cells, ongoing clinical trials have employed cell-sorting strategies to boost the endogenous regenerative potential. This next-generation strategy either purifies unique populations of autologous or allogeneic progenitors [6] or achieves anatomical matching with propagation of resident cardiac stem cells [7]. Furthermore, identification of signals that govern cardiogenic specification facilitated the development of cardiopoiesis technology used to boost the regenerative impact of patient-derived stem cells [8].Table 1. Optimization efforts for the cardiac regenerative paradigmCell populationsOptimization strategyExpected impactProgress to datePurified cells, mesenchymal stem cells (MSCs), and endothelial progenitor cells (EPCs)Well-defined populationRepair effect of well-defined progenitors with potential allogeneic off-shelf therapy• Synergistic use of MSCs and cardiac progenitor cells (CPCs) is currently under investigation• Strong signals of benefit with EPCsCPCsAnatomical matchingBoosting intrinsic regenerative pool• Two phase II trials completed• Further studies with allogenic populations are under wayFunctional lineage matchingGrowth factor-guided lineage specificationBoosting paracrine effects together with intrinsic regenerative pool• Early signals of efficacy• This paradigm is under phase III investigationDelivery techniquesNeedle designIncreased retention and cell survivalCurved needle design shown to improve the cell retentionProcedural guidanceMyocardial niche modificationEnhanced homing signalingBoosting cell retention and endogenous repairParacrine influence of progenitors achieves enhanced stem cell content in the perivascular stem cell niche within the heartTissue engineeringBioscaffoldsFacilitate cell survival and endogenous repairSignificant advances in tissue processing and three-dimensional printing have paved the way for cell-free approaches for cardiac regeneration

Use of acellular approaches to achieve cardiac regeneration has evolved out of stem cell science but aims to achieve regeneration without the cell-to-cell variability and high manufacturing costs associated with cell therapy. Here, delivery of restorative exogenous signals through the use of scaffolds, proteins, or microRNA aims to boost intrinsic repair function of the heart by modulating the interplay between reactive inflammatory response and stem cell recruitment into the ischemic tissue. In this regard, manipulation of the myocardial microenvironmental appears to boost the stem cell recruitment into the perivascular niche and enhance the efficacy of autologous stem cell delivery [9].

Beyond cell-based or cell-free strategies that boost the endogenous myocardial response, procedural optimization to augment the retention of biologics relies on the development of novel delivery devices or methods to optimize procedural guidance [10]. Alternatively, engineered biomaterial scaffolds may help to overcome challenges currently attributed to cell retention or host tissue niche modification [2]. The present study provides a biological proof of concept that pericardial scaffolds can integrate with host tissue in the setting of acute myocardial infarction, creating neurovascular networks in the epicardial space. Although implementation of this particular approach early after infarction is clinically impractical, integration in chronic models of heart failure may provide a novel approach to regeneration. The observations in this study pave the road for further studies to explore the functional effects of bioscaffold alone or in combination with various cell types.

Conclusion

Cardiac tissue engineering seizing upon the advances of cellular, molecular, and biomaterial development is shaping the next generation of the regenerative paradigm and thereby fostering disruptive curative treatments in heart failure.