Integrated Functional and scRNA-Seq Analyses Reveal Convergence of M-CSF– and GM-CSF–Derived Macrophages Following IL-27 Polarization
Tomozumi Imamichi, Jun Yang, Qian Chen, Udeshika Kariyawasam, Mayra Marquez, Jeanette Higgins, Jordan Metz, Homa Nath Sharma, Michael W. Baseler, Hongyan Sui
TL;DR
This study shows that IL-27 can reprogram two types of macrophages into a similar state, suggesting a key role for IL-27 in macrophage plasticity.
Contribution
The study reveals that IL-27 induces a convergent phenotype in M-CSF- and GM-CSF-derived macrophages.
Findings
IL-27-polarized M-Mac and GM-Mac show similar transcriptional and functional profiles.
IL-27 treatment suppresses HIV replication and enhances CD38 expression and autophagy in macrophages.
Phagocytic activity is reduced in IL-27-polarized macrophages compared to controls.
Abstract
What are the main findings? Two distinct macrophage subsets—M-CSF– and GM-CSF–differentiated monocyte-derived macrophages (MDMs)—are reprogrammed by IL-27 into a convergent phenotype Two distinct macrophage subsets—M-CSF– and GM-CSF–differentiated monocyte-derived macrophages (MDMs)—are reprogrammed by IL-27 into a convergent phenotype What are the implications of the main findings? IL-27 functions as a convergent polarization cytokine.IL-27 may play a central role in regulating macrophage plasticity. IL-27 functions as a convergent polarization cytokine. IL-27 may play a central role in regulating macrophage plasticity. Macrophages differentiated with macrophage colony-stimulating factor (M-CSF) (M-Mac) are widely used as an experimental model. Interleukin 27 (IL-27)-polarized M-Mac (27M-Mac) suppresses HIV replication; however, the effects of IL-27 polarization on…
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Taxonomy
TopicsImmune cells in cancer · HIV Research and Treatment · Autophagy in Disease and Therapy