The phytohormone indole-3-acetic acid induces quorum sensing signal DSF turnover via a positive feedback biosynthetic loop in the phytopathogen Xanthomonas campestris
Si-Nan Li, Ming-Lei Zhang, Ying Cui, Lin Li, Chitti Thawai, Lian Jiang, Dong-Lan Tian, Yu-Cheng Gu, Ya-Wen He, Lian Zhou, Kai Song
TL;DR
This study shows that the plant hormone IAA helps the bacteria Xanthomonas campestris regulate its communication signals during infection.
Contribution
The study reveals a new role for IAA in modulating bacterial quorum sensing through a positive feedback loop.
Findings
IAA induces DSF turnover via RpfB-dependent mechanisms.
IAA upregulates F0F1 ATP synthases and HepABCD to modulate pH-dependent DSF turnover.
IAA downregulates IaepABCDE to modulate pH-independent DSF turnover.
Abstract
Indole-3-acetic acid (IAA) accumulates in host plants following infection by Xanthomonas campestris pv. campestris (Xcc), the causal agent of cruciferous black rot. How exposure to IAA affects the invading Xcc remains unclear. Here, we demonstrate that either exogenous addition of IAA or endogenous production of IAA induced turnover of the quorum sensing (QS) signal diffusible signaling factor (DSF) in a RpfB-dependent manner. IAA addition prevented the cytoplasmic and culture pH decline. Transcriptomic analyses revealed four IAA-regulated gene clusters. Specifically, IAA induced the expression of trpB-A, enhancing tryptophan biosynthesis and intracellular IAA accumulation, and thereby establishing a self-reinforcing synthesis loop. IAA upregulated F0F1 ATP synthases and a resistance-nodulation-cell division (RND)-family efflux pump HepABCD to induce a pH-dependent DSF turnover.…
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Taxonomy
TopicsBacterial biofilms and quorum sensing · Plant-Microbe Interactions and Immunity · Plant Pathogenic Bacteria Studies