Single-Armed Longitudinal Intussusception Vasoepididymostomy in an Ex Vivo Rat Model With Video Demonstration
Yelena Akelina, Jan B Lombaard, Nicholas J Coleman, Joseph Abboud

TL;DR
This paper introduces a step-by-step video-guided microsurgical technique for vasoepididymostomy in rats using single-armed sutures, aiming to improve training and reproducibility.
Contribution
The novel contribution is a standardized, video-demonstrated SA-LIVE technique using single-armed sutures for microsurgical training in an ex vivo rat model.
Findings
A step-by-step SA-LIVE technique is presented with a video to aid microsurgical training.
The method uses readily available single-armed sutures, reducing reliance on double-armed sutures.
The protocol is designed for an ex vivo rat model to standardize longitudinal intussusception.
Abstract
Microsurgical vasoepididymostomy (VE) is a technically demanding procedure that benefits from deliberate, reproducible skills training. Practice opportunities may be limited by the cost and availability of double-armed microsutures used in traditional two-needle intussusception techniques. Single-armed longitudinal intussusception vasoepididymostomy (SA-LIVE) reduces reliance on double-armed sutures. Although SA-LIVE has been described in prior publications, still images alone may not fully capture the sequence and microsurgical nuances; a video demonstration can improve clarity for learners. We present a step-by-step SA-LIVE technique for an ex vivo rat testis training model, using readily available single-armed 10-0 and 9-0 nylon sutures. The protocol standardizes the longitudinal intussusception sequence and is paired with a video demonstration illustrating each step to aid…
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Taxonomy
TopicsTesticular diseases and treatments · Sexual Differentiation and Disorders · Sperm and Testicular Function
