# Single-Armed Longitudinal Intussusception Vasoepididymostomy in an Ex Vivo Rat Model With Video Demonstration

## TL;DR

This paper introduces a step-by-step video-guided microsurgical technique for vasoepididymostomy in rats using single-armed sutures, aiming to improve training and reproducibility.

## Contribution

The novel contribution is a standardized, video-demonstrated SA-LIVE technique using single-armed sutures for microsurgical training in an ex vivo rat model.

## Key findings

- A step-by-step SA-LIVE technique is presented with a video to aid microsurgical training.
- The method uses readily available single-armed sutures, reducing reliance on double-armed sutures.
- The protocol is designed for an ex vivo rat model to standardize longitudinal intussusception.

## Abstract

Microsurgical vasoepididymostomy (VE) is a technically demanding procedure that benefits from deliberate, reproducible skills training. Practice opportunities may be limited by the cost and availability of double-armed microsutures used in traditional two-needle intussusception techniques. Single-armed longitudinal intussusception vasoepididymostomy (SA-LIVE) reduces reliance on double-armed sutures. Although SA-LIVE has been described in prior publications, still images alone may not fully capture the sequence and microsurgical nuances; a video demonstration can improve clarity for learners. We present a step-by-step SA-LIVE technique for an ex vivo rat testis training model, using readily available single-armed 10-0 and 9-0 nylon sutures. The protocol standardizes the longitudinal intussusception sequence and is paired with a video demonstration illustrating each step to aid comprehension and reproducibility. This technical report is intended as a microsurgical training resource and does not include comparative outcome data.