Innovative Membrane Purification of Plant-Based Lipase from Flaxseed Using Graphene Oxide
Nicole Novelli do Nascimento, Paulo Waldir Tardioli, Rosângela Bergamasco, Angélica Marquetotti Salcedo Vieira
TL;DR
Researchers developed a new membrane method to efficiently purify high-activity lipase from flaxseed, making it a sustainable industrial enzyme.
Contribution
A novel membrane using graphene oxide and polyethylenimine significantly improves flaxseed lipase purification and reusability.
Findings
Lipase activity peaks after 4 hours of flaxseed germination.
The membrane purification method increased specific activity by 11.6-fold.
The membrane can be reused for at least two cycles with 74.38% flow recovery.
Abstract
Plant-derived lipases offer a cost-effective and sustainable alternative to microbial and animal enzymes for industrial use. Flaxseed is a promising source due to its high lipase activity during germination, although efficient extraction of the active enzyme remains challenging. This study evaluates optimized extraction and purification strategies for lipase from germinated, defatted flaxseed. Maximum specific activity was observed after 4 h of germination. Purification using a membrane modified with polyethylenimine (PEI) and graphene oxide (GO) nanoparticles functionalized with tannic acid (TA) significantly enhanced performance, increasing specific activity by approximately 11.6-fold. Membrane characterization showed reduced pore size and improved antifouling behavior, allowing reuse for at least two cycles with a 74.38% flow recovery. The purified lipase demonstrated thermal…
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Taxonomy
TopicsEnzyme Catalysis and Immobilization · Membrane Separation Technologies · Proteins in Food Systems