# Innovative Membrane Purification of Plant-Based Lipase from Flaxseed Using Graphene Oxide

## TL;DR

Researchers developed a new membrane method to efficiently purify high-activity lipase from flaxseed, making it a sustainable industrial enzyme.

## Contribution

A novel membrane using graphene oxide and polyethylenimine significantly improves flaxseed lipase purification and reusability.

## Key findings

- Lipase activity peaks after 4 hours of flaxseed germination.
- The membrane purification method increased specific activity by 11.6-fold.
- The membrane can be reused for at least two cycles with 74.38% flow recovery.

## Abstract

Plant-derived lipases offer a cost-effective and sustainable
alternative
to microbial and animal enzymes for industrial use. Flaxseed is a
promising source due to its high lipase activity during germination,
although efficient extraction of the active enzyme remains challenging.
This study evaluates optimized extraction and purification strategies
for lipase from germinated, defatted flaxseed. Maximum specific activity
was observed after 4 h of germination. Purification using a membrane
modified with polyethylenimine (PEI) and graphene oxide (GO) nanoparticles
functionalized with tannic acid (TA) significantly enhanced performance,
increasing specific activity by approximately 11.6-fold. Membrane
characterization showed reduced pore size and improved antifouling
behavior, allowing reuse for at least two cycles with a 74.38% flow
recovery. The purified lipase demonstrated thermal stability between
25 and 60 °C. Overall, the proposed membrane-based approach enables
the efficient production of high-activity flaxseed lipase, reinforcing
its potential as a sustainable and economically viable industrial
biocatalyst.