Benchtop NMR signal enhancement of metabolites in urine extract using SABRE
Simon Fleischer, Jing Yang, Kerti Ausmees, Indrek Reile, Neil MacKinnon, Jan Gerrit Korvink, Sören Lehmkuhl
TL;DR
This paper shows how to boost NMR signals of urine metabolites using a technique called SABRE, enabling faster and more sensitive detection with minimal equipment.
Contribution
The novel contribution is demonstrating automated SABRE hyperpolarization for enhanced metabolite detection in urine using a benchtop NMR.
Findings
SABRE hyperpolarization significantly enhances NMR signals of urinary nicotinamide.
Standard addition quantification of nicotinamide was successfully demonstrated.
An automated setup improved sensitivity for multi-scan SABRE experiments.
Abstract
Metabolites in a urine extract are signal-enhanced by SABRE††SABRE: Signal Amplification By Reversible Exchange. hyperpolarization and detected using a benchtop NMR spectrometer. Quantification by standard addition is demonstrated for endogenic urinary nicotinamide (vitamin B3). Even higher sensitivity is achieved in an automated setup for multi-scan SABRE experiments. This hyperpolarization scheme is able to expedite biomarker detection and quantification, while maintaining low infrastructure requirements. SABRE: Signal Amplification By Reversible Exchange. Enhancing NMR signals of urinary metabolites using SABRE hyperpolarization in an automated benchtop-scale setup.
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsAdvanced NMR Techniques and Applications · Metabolomics and Mass Spectrometry Studies · NMR spectroscopy and applications