# Benchtop NMR signal enhancement of metabolites in urine extract using SABRE

## TL;DR

This paper shows how to boost NMR signals of urine metabolites using a technique called SABRE, enabling faster and more sensitive detection with minimal equipment.

## Contribution

The novel contribution is demonstrating automated SABRE hyperpolarization for enhanced metabolite detection in urine using a benchtop NMR.

## Key findings

- SABRE hyperpolarization significantly enhances NMR signals of urinary nicotinamide.
- Standard addition quantification of nicotinamide was successfully demonstrated.
- An automated setup improved sensitivity for multi-scan SABRE experiments.

## Abstract

Metabolites in a urine extract are signal-enhanced by SABRE††SABRE: Signal Amplification By Reversible Exchange. hyperpolarization and detected using a benchtop NMR spectrometer. Quantification by standard addition is demonstrated for endogenic urinary nicotinamide (vitamin B3). Even higher sensitivity is achieved in an automated setup for multi-scan SABRE experiments. This hyperpolarization scheme is able to expedite biomarker detection and quantification, while maintaining low infrastructure requirements.

SABRE: Signal Amplification By Reversible Exchange.

Enhancing NMR signals of urinary metabolites using SABRE hyperpolarization in an automated benchtop-scale setup.