Resveratrol Alleviates Effects of LPS on Estrogen Synthesis, Oxidative Stress, Inflammation, and Pyroptosis of Goat Granulosa Cells by Activating the PPARG/NRF2/HO-1 Signaling Pathway
Jie Zhao, Xianyi Zhou, Zhen Cang, Xin Liu, Muhammad Tariq, Dagan Mao
TL;DR
Resveratrol helps protect goat granulosa cells from LPS damage by activating a specific signaling pathway, reducing inflammation and oxidative stress.
Contribution
The study reveals a novel mechanism by which resveratrol alleviates LPS-induced injury via the PPARG/NRF2/HO-1 pathway in goat granulosa cells.
Findings
Resveratrol reduced LPS-induced oxidative stress and inflammation in goat granulosa cells.
Resveratrol's protective effects were blocked by a PPARG antagonist, confirming pathway involvement.
The PPARG/NRF2/HO-1 pathway was identified as a key mediator of resveratrol's actions.
Abstract
This study aims to investigate the effect and mechanism of resveratrol (RES) on lipopolysaccharide (LPS)-induced injury in goat granulosa cells (GCs). First, the appropriate time and concentration were screened for LPS (4 μg/mL, 12 h), RES (1 μM, 6 h), and GW9662 (an antagonist of PPARG, 1 μM, 12 h) through CCK8 and RT-qPCR. Then, cells were treated with LPS, RES, or/and GW9662, to examine steroidogenesis, inflammation, oxidative stress, and pyroptosis by RIA, RT-qPCR, WB, flow cytometry, and IF, respectively. Results showed that RES inhibited LPS-induced increases in MDA content, ROS production, gene expressions of IL-1β, NLRP3, Caspase1, and GSDMD, as well as protein levels of IL-1β, and GSDMD, accompanied by decreases in SOD activity, T-AOC and E2 content, gene expressions of SOD, CYP19A1, and HSD3B, and protein levels of SOD and HSD3B. Furthermore, RES inhibited LPS-induced…
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Taxonomy
TopicsSirtuins and Resveratrol in Medicine · Inflammasome and immune disorders · Genomics, phytochemicals, and oxidative stress