The Dynamic Effects of Transposon Insertions on AuxRP1 Transcription During Vegetative Development in Zea mays

TL;DR

This study explores how transposon insertions affect the transcription of the AuxRP1 gene during the growth stages of corn plants.

Contribution

The study reveals stage-specific effects of transposon insertions on AuxRP1 transcription and related gene pathways in corn.

Findings

Transposon insertions caused decreased or unchanged AuxRP1 transcription at the juvenile stage but increased it at the transition/adult stage.

Transposon insertions affect related genes like TSB2C and Yucca2, influencing biosynthetic pathways.

The effects of transposon insertions are stage-specific and impact auxin signaling and stalk rot resistance.

Abstract

The
AuxRP1
gene (
Zm00001eb053610
) is involved in the auxin signaling pathway and stalk rot resistance in
Zea mays
. In this study, we examined four transposon insertion lines (UFMu-03429, UFMu-00414, UFMu-08200, and AcDs-00676) targeting
AuxRP1
. Transcription of
AuxRP1
of the insertion lines either decreased or remained unchanged at the juvenile (V3) stage but increased significantly at the transition/adult (V6) stage. We also analyzed its upstream gene
TSB2C
and downstream gene
Yucca2
. Our results show that transposon insertions can induce stage-specific changes in gene expression that affect related biosynthetic pathways.

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