Chromosomal and extrachromosomal replicons of a multidrug-resistant zoonotic Staphylococcus ureilyticus strain RSM-JSascm isolated from cow milk in Western Uganda, sub-Saharan Africa

TL;DR

This paper reports the genome of a multidrug-resistant Staphylococcus ureilyticus strain from cow milk in Uganda, revealing its chromosomal and plasmid structure and resistance genes.

Contribution

The study provides the first detailed genomic analysis of a multidrug-resistant Staphylococcus ureilyticus isolate from sub-Saharan Africa.

Findings

The isolate's chromosome contains 2,534 coding sequences and multiple RNA genes.

Three plasmids were identified, carrying antimicrobial resistance genes.

CRISPR arrays were found in the chromosome, suggesting potential for adaptive immunity.

Abstract

Staphylococcus ureilyticus is a less-studied pathogen with zoonotic and antimicrobial resistance (AMR) potential. We sequenced a bovine isolate and recovered four replicons, a chromosome with 2,534 coding sequences (CDS), 51 transfer RNAs (tRNA), four ribosomal RNA (rRNA) genes, and two CRISPR arrays, along with three plasmids, harboring AMR genes.

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