Genotyping of the gene polymorphisms FASL 844C/T and FAS 670A/G in patients with idiopathic infertility in Iraq

TL;DR

This study examines how gene variations in FAS and FASL may contribute to male infertility in Iraq.

Contribution

The study identifies specific gene polymorphisms linked to male infertility in Iraqi populations.

Findings

FAS-670A/G polymorphism is significantly associated with azoospermia and oligospermia.

FASL-844C/T polymorphism shows significant links to male infertility subtypes.

Homozygous mutant genotypes GG (FAS) and TT (FASL) are risk factors for infertility.

Abstract

The failure to conceive following 12 months of unprotected intercourse is referred to as infertility. About 35% of the instances include women and 30% involve men.

The aim of this study was to investigate the gene polymorphisms FASL-844C/T and FAS-670A/G in the promoter region to evaluate susceptibility to male infertility.

We have investigated single-nucleotide polymorphisms of FAS-670A/G gene and FASL-844C/T gene in 100 subjects. A total of 50 male patients with infertility constituted the infertility group and another group consisting of 50 apparently healthy individuals comprised the control group.

The prevalence rate of FAS-670A/G gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype GG (p=0.014 and p=0.043), and mutant allele G (p=0.001 and p=0.004), respectively. Also, FASL-844C/T gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype TT (p=0.032 and p=0.032), and mutant allele T (p=0.007 and p=0.002), respectively.

FAS homozygous mutant genotype (GG) and FASL homozygous mutant genotype (TT) act as etiologic factors in male infertility.