# Genotyping of the gene polymorphisms FASL 844C/T and FAS 670A/G in patients with idiopathic infertility in Iraq

## TL;DR

This study examines how gene variations in FAS and FASL may contribute to male infertility in Iraq.

## Contribution

The study identifies specific gene polymorphisms linked to male infertility in Iraqi populations.

## Key findings

- FAS-670A/G polymorphism is significantly associated with azoospermia and oligospermia.
- FASL-844C/T polymorphism shows significant links to male infertility subtypes.
- Homozygous mutant genotypes GG (FAS) and TT (FASL) are risk factors for infertility.

## Abstract

The failure to conceive following 12 months of unprotected intercourse is referred to as infertility. About 35% of the instances include women and 30% involve men.

The aim of this study was to investigate the gene polymorphisms FASL-844C/T and FAS-670A/G in the promoter region to evaluate susceptibility to male infertility.

We have investigated single-nucleotide polymorphisms of FAS-670A/G gene and FASL-844C/T gene in 100 subjects. A total of 50 male patients with infertility constituted the infertility group and another group consisting of 50 apparently healthy individuals comprised the control group.

The prevalence rate of FAS-670A/G gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype GG (p=0.014 and p=0.043), and mutant allele G (p=0.001 and p=0.004), respectively. Also, FASL-844C/T gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype TT (p=0.032 and p=0.032), and mutant allele T (p=0.007 and p=0.002), respectively.

FAS homozygous mutant genotype (GG) and FASL homozygous mutant genotype (TT) act as etiologic factors in male infertility.