A Pilot Study on Qualitative Metabolomics to Characterize Lewis Lung Carcinoma in Mice

TL;DR

This study uses metabolomics to identify changes in mouse lung cancer, highlighting potential biomarkers for cancer research.

Contribution

The study presents a novel metabolomic profile of Lewis lung carcinoma in mice using NMR spectroscopy.

Findings

Metabolomic analysis revealed changes in carbohydrate and amino acid metabolism in LLC tumors.

Elevated lactate levels indicate hypoxia in lung cancer tissues.

The LLC metabolomic profile may serve as a valuable biomarker for lung cancer research.

Abstract

Metabolomics is a powerful tool that can be used to identify different stages in cancer development. In this study, the metabolomic profile of Lewis lung carcinoma (LLC) was characterized in C57BL/6 mice bearing LLC tumors. Magnetic resonance spectroscopy (nuclear magnetic resonance—NMR) was applied using a 400 MHz 1H NMR spectrometer. Two types of metabolites (polar and non-polar) were identified on LLC based on the analysis of methanol/water and chloroform extracts collected from lung cancer samples in mice. The investigated metabolomics show that the neoplastic processes of growing LLC on mice may affect carbohydrate; alanine and glutamate; leucine and isoleucine; lysine; creatine; and choline metabolism, whereas hypoxia states were identified due to elevated lactate in lung cancer tissues. The metabolomic profile of Lewis lung carcinoma could be considered to be a valuable biomarker in translational lung cancer research.

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