# A Pilot Study on Qualitative Metabolomics to Characterize Lewis Lung Carcinoma in Mice

## TL;DR

This study uses metabolomics to identify changes in mouse lung cancer, highlighting potential biomarkers for cancer research.

## Contribution

The study presents a novel metabolomic profile of Lewis lung carcinoma in mice using NMR spectroscopy.

## Key findings

- Metabolomic analysis revealed changes in carbohydrate and amino acid metabolism in LLC tumors.
- Elevated lactate levels indicate hypoxia in lung cancer tissues.
- The LLC metabolomic profile may serve as a valuable biomarker for lung cancer research.

## Abstract

Metabolomics is a powerful tool that can be used to identify different stages in cancer development. In this study, the metabolomic profile of Lewis lung carcinoma (LLC) was characterized in C57BL/6 mice bearing LLC tumors. Magnetic resonance spectroscopy (nuclear magnetic resonance—NMR) was applied using a 400 MHz 1H NMR spectrometer. Two types of metabolites (polar and non-polar) were identified on LLC based on the analysis of methanol/water and chloroform extracts collected from lung cancer samples in mice. The investigated metabolomics show that the neoplastic processes of growing LLC on mice may affect carbohydrate; alanine and glutamate; leucine and isoleucine; lysine; creatine; and choline metabolism, whereas hypoxia states were identified due to elevated lactate in lung cancer tissues. The metabolomic profile of Lewis lung carcinoma could be considered to be a valuable biomarker in translational lung cancer research.