Genetic Mapping of prod E.3.3 , a New Lethal Allele of prod

TL;DR

This paper describes a new lethal mutation in the prod gene that causes eye development defects in fruit flies.

Contribution

The study identifies a novel allele of prod, E.3.3, and links it to developmental defects through genetic and genomic analysis.

Findings

The E.3.3 mutation disrupts the functional region of the Prod protein.

E.3.3 causes eye development defects likely due to disrupted chromatid separation.

Genome sequencing and complementation mapping confirmed E.3.3 as a new allele of prod.

Abstract

The
E.3.3
mutation was generated in a Flp/FRT EMS screen for conditional mutations that cause growth and developmental defects in a genetic background that blocks apoptosis. The mutations were conditional, based on the

Dark
82

allele being present on the starting chromosome, and blocking canonical apoptosis in a homozygous state. The
E.3.3
mosaic eyes exhibit defects in eye development including patches of rough eye and irregular surface structure. Whole Genome Sequencing and complementation mapping revealed
E.3.3
as an allele of
prod
. Prod is a DNA-binding protein that binds satellite repeats and is involved in chromocenter formation during mitosis. Here we present a novel allele of
prod
,

prod
E.3.3

, that disrupts the functional region of the Prod protein resulting in disruption of typical eye structure, likely due to disruption of chromatid separation during development.

Figures