Chromosome-level assembly and evolution analysis of the Trichosanthes truncata genome
Ying Hu, Xiaomei Wei, Zhuannan Chu, Fan Wei, Yude Peng, Baoyou Huang, Ling Dong, Kunhua Wei, Weiwen Li
TL;DR
This paper presents a high-quality genome assembly for the medicinal plant Trichosanthes truncata, aiding in understanding its bioactive compound biosynthesis and plant genomics.
Contribution
The study provides the first chromosome-level genome assembly of Trichosanthes truncata using advanced sequencing and mapping technologies.
Findings
A 637.41 Mb genome was assembled with an N50 of 57.24 Mb and divided into 11 pseudochromosomes.
The assembly includes 36,172 protein-coding genes and 97.21 Mb of repetitive sequences.
The genome will help study the molecular mechanisms of bioactive compound biosynthesis in this medicinal plant.
Abstract
Trichosanthes truncata C. B. Clarke, an important medicinal plant, is a dioecious plant belonging to the Cucurbitaceae family. This study presents a chromosomal-level reference genome assembly for T. truncata. Through the integration of PacBio high-fidelity sequencing and high-throughput chromosome conformation capture technology, a final genome sequence of 637.41 Mb was assembled, with an N50 of 57.24 Mb and consisting of 11 pseudochromosomes. Additionally, 97.21 Mb of repetitive sequences and 36,172 protein-coding genes were annotated. This high-quality genome assembly is of utmost significance for studying the molecular mechanisms underlying the biosynthesis of bioactive compounds. Furthermore, this study provided valuable insights into plant comparative genomics research.
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Taxonomy
TopicsChromosomal and Genetic Variations · Genomics and Phylogenetic Studies · Plant Pathogenic Bacteria Studies