# Chromosome-level assembly and evolution analysis of the Trichosanthes truncata genome

## TL;DR

This paper presents a high-quality genome assembly for the medicinal plant Trichosanthes truncata, aiding in understanding its bioactive compound biosynthesis and plant genomics.

## Contribution

The study provides the first chromosome-level genome assembly of Trichosanthes truncata using advanced sequencing and mapping technologies.

## Key findings

- A 637.41 Mb genome was assembled with an N50 of 57.24 Mb and divided into 11 pseudochromosomes.
- The assembly includes 36,172 protein-coding genes and 97.21 Mb of repetitive sequences.
- The genome will help study the molecular mechanisms of bioactive compound biosynthesis in this medicinal plant.

## Abstract

Trichosanthes truncata C. B. Clarke, an important medicinal plant, is a dioecious plant belonging to the Cucurbitaceae family. This study presents a chromosomal-level reference genome assembly for T. truncata. Through the integration of PacBio high-fidelity sequencing and high-throughput chromosome conformation capture technology, a final genome sequence of 637.41 Mb was assembled, with an N50 of 57.24 Mb and consisting of 11 pseudochromosomes. Additionally, 97.21 Mb of repetitive sequences and 36,172 protein-coding genes were annotated. This high-quality genome assembly is of utmost significance for studying the molecular mechanisms underlying the biosynthesis of bioactive compounds. Furthermore, this study provided valuable insights into plant comparative genomics research.