Efficient Knock-in by CRISPR/Cas9-mediated homology-directed repair in Caenorhabditis briggsae
Jiaonv Ma, Wangyan Zhou, Wenhua Shao, Yongbin Li
TL;DR
This study shows that using high concentrations of donor vectors improves CRISPR/Cas9 knock-in efficiency in the nematode Caenorhabditis briggsae.
Contribution
The novel finding is that high donor vector concentration enhances homology-directed repair in C. briggsae.
Findings
High concentration HDR donor vectors significantly improve knock-in efficiency in C. briggsae.
CRISPR/Cas9 knock-in in C. briggsae faces challenges, but this study provides a solution to enhance it.
Abstract
The CRISPR/Cas9 technology opens new avenues for detailed genetic exploration and comparative genomics. However, the current application of CRISPR/Cas9 in C. briggsae especially for homology-directed repair (HDR) knock-in (KI) still encounter significant challenges. In this study, we demonstrate that employing high concentration HDR donor vectors significantly improves KI efficiency in C. briggsae .
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Taxonomy
TopicsCRISPR and Genetic Engineering · Genetics and Neurodevelopmental Disorders · Pluripotent Stem Cells Research