# Efficient Knock-in by CRISPR/Cas9-mediated homology-directed repair in Caenorhabditis briggsae

## TL;DR

This study shows that using high concentrations of donor vectors improves CRISPR/Cas9 knock-in efficiency in the nematode Caenorhabditis briggsae.

## Contribution

The novel finding is that high donor vector concentration enhances homology-directed repair in C. briggsae.

## Key findings

- High concentration HDR donor vectors significantly improve knock-in efficiency in C. briggsae.
- CRISPR/Cas9 knock-in in C. briggsae faces challenges, but this study provides a solution to enhance it.

## Abstract

The CRISPR/Cas9 technology opens new avenues for detailed genetic exploration and comparative genomics. However, the current application of CRISPR/Cas9 in
C. briggsae
especially for homology-directed repair (HDR) knock-in (KI) still encounter significant challenges. In this study, we demonstrate that employing high concentration HDR donor vectors significantly improves KI efficiency in
C. briggsae
.