The correlation between the MYBL2/CDCA8 signaling pathway of malignant melanoma
Chen Wu, Jiahui Jiang, Chao Ci
TL;DR
This study shows that the MYBL2 gene promotes melanoma cell migration and invasion by regulating CDCA8 expression.
Contribution
The novel finding is that MYBL2 promotes melanoma progression by regulating CDCA8 expression.
Findings
MYBL2 knockdown reduced melanoma cell migration and invasion in vitro.
MYBL2 knockdown decreased tumor volume and CDCA8 expression in vivo.
MYBL2 and CDCA8 expression levels are positively correlated.
Abstract
Investigating the effects of MYB proto-oncogene like 2 (MYBL2)-mediated regulation of Cell division cycle associated 8 (CDCA8) expression on the biological activity of cutaneous malignant melanoma cells. A375 cells with MYBL2 and CDCA8 overexpression and knockdown were evaluated using migration, invasion, and proliferation assays. Besides, cell apoptosis was quantified by flow cytometry. To investigate the tumorigenic effects of MYBL2 knockdown in vivo, A375 cells with MYBL2 knockdown were injected in BALB/C nude mice. The levels of MYBL2 and CDCA8 gene expression were notably elevated in A375 cells in comparison to HaCat cells (P < 0.05). Downregulation of MYBL2 led to a notable reduction in the migratory and invasive capability of A375 cells in vitro (P < 0.001). On the contrary, overexpression of MYBL2 enhanced migration and invasion ability (P < 0.001). There existed a positive…
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Taxonomy
TopicsMelanoma and MAPK Pathways · CAR-T cell therapy research · Cell Adhesion Molecules Research