# The correlation between the MYBL2/CDCA8 signaling pathway of malignant melanoma

## TL;DR

This study shows that the MYBL2 gene promotes melanoma cell migration and invasion by regulating CDCA8 expression.

## Contribution

The novel finding is that MYBL2 promotes melanoma progression by regulating CDCA8 expression.

## Key findings

- MYBL2 knockdown reduced melanoma cell migration and invasion in vitro.
- MYBL2 knockdown decreased tumor volume and CDCA8 expression in vivo.
- MYBL2 and CDCA8 expression levels are positively correlated.

## Abstract

Investigating the effects of MYB proto-oncogene like 2 (MYBL2)-mediated regulation of Cell division cycle associated 8 (CDCA8) expression on the biological activity of cutaneous malignant melanoma cells.

A375 cells with MYBL2 and CDCA8 overexpression and knockdown were evaluated using migration, invasion, and proliferation assays. Besides, cell apoptosis was quantified by flow cytometry. To investigate the tumorigenic effects of MYBL2 knockdown in vivo, A375 cells with MYBL2 knockdown were injected in BALB/C nude mice.

The levels of MYBL2 and CDCA8 gene expression were notably elevated in A375 cells in comparison to HaCat cells (P < 0.05). Downregulation of MYBL2 led to a notable reduction in the migratory and invasive capability of A375 cells in vitro (P < 0.001). On the contrary, overexpression of MYBL2 enhanced migration and invasion ability (P < 0.001). There existed a positive correlation between CDCA8 and MYBL2 gene and protein expression levels after overexpression or knockdown of MYBL2 (P < 0.001). In the in vivo tumorigenic study, the MYBL2 knockdown group displayed a substantial decrease in tumor volume (P < 0.01) and exhibited decreased CDCA8 expression in tumors in comparison to the control group.

We arrived at such a conclusion that MYBL2 promoted the migration, invasion and proliferation ability of cutaneous malignant melanoma cells by targeted regulation of CDCA8 expression in this study.