Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence

TL;DR

This paper identifies reliable antibodies for studying the SYK protein, which is linked to inflammation and diseases like Alzheimer's.

Contribution

The study evaluates thirteen commercial SYK antibodies using standardized protocols to identify high-performing ones for common research techniques.

Findings

Thirteen commercial SYK antibodies were tested for Western Blot, immunoprecipitation, and immunofluorescence.

Several high-performing antibodies were identified based on comparisons with knockout and parental cell lines.

The findings aim to improve reproducibility in SYK-related research and its role in neuroinflammation and Alzheimer's.

Abstract

Tyrosine-protein kinase SYK, encoded by the
SYK gene, is a non-receptor type protein kinase which mediates immune signal transduction through immunoreceptors. Tyrosine-protein kinase SYK expression has been associated with the development of various inflammatory diseases, cancer and neurodegenerative conditions. The reproducibility of tyrosine-protein kinase SYK research would help elucidate the mechanism in which it causes neuroinflammation as well as its potential as a novel target to treat Alzheimer’s disease. This would be facilitated with the availability of high-quality tyrosine-protein kinase SYK. In this study, we characterized thirteen tyrosine-protein kinase SYK commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

Figures