# Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence

## TL;DR

This paper identifies reliable antibodies for studying the SYK protein, which is linked to inflammation and diseases like Alzheimer's.

## Contribution

The study evaluates thirteen commercial SYK antibodies using standardized protocols to identify high-performing ones for common research techniques.

## Key findings

- Thirteen commercial SYK antibodies were tested for Western Blot, immunoprecipitation, and immunofluorescence.
- Several high-performing antibodies were identified based on comparisons with knockout and parental cell lines.
- The findings aim to improve reproducibility in SYK-related research and its role in neuroinflammation and Alzheimer's.

## Abstract

Tyrosine-protein kinase SYK, encoded by the
SYK gene, is a non-receptor type protein kinase which mediates immune signal transduction through immunoreceptors. Tyrosine-protein kinase SYK expression has been associated with the development of various inflammatory diseases, cancer and neurodegenerative conditions. The reproducibility of tyrosine-protein kinase SYK research would help elucidate the mechanism in which it causes neuroinflammation as well as its potential as a novel target to treat Alzheimer’s disease. This would be facilitated with the availability of high-quality tyrosine-protein kinase SYK. In this study, we characterized thirteen tyrosine-protein kinase SYK commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.