Hepatotoxicity assessment of innovative nutritional supplements based on olive-oil formulations enriched with natural antioxidants
Sofia I. Prodromou, Fani Chatzopoulou, Aikaterini Saiti, Alexandros Giannopoulos-Dimitriou, Loukia A. Koudoura, Anastasia A. Pantazaki, Dimitrios Chatzidimitriou, Vasilis Vasiliou, Ioannis S. Vizirianakis
TL;DR
This study evaluates the safety and benefits of olive-oil supplements with natural antioxidants for reducing oxidative stress and inflammation in diseases like Alzheimer's.
Contribution
The study introduces innovative olive-oil formulations and evaluates their hepatotoxicity and molecular effects using targeted molecular analysis.
Findings
Minimal cellular toxicity was observed in HepG2 cells exposed to the formulations.
The supplements showed a significant positive impact on metabolic pathways related to oxidative stress.
Natural antioxidant delivery modes were contrasted with chemical forms to assess cell toxicity.
Abstract
This study focuses on the assessment of extra virgin olive-oil and olive fruit-based formulations enriched with natural antioxidants as potential nutritional supplements for alleviating symptoms and long-term consequences of illnesses whose molecular pathophysiology is affected by oxidative stress and inflammation, such as Alzheimer’s disease (AD). Besides evaluating cell viability and proliferation capacity of human hepatocellular carcinoma HepG2 cells exposed to formulations in culture, hepatotoxicity was also considered as an additional safety measure using quantitative real-time PCR on RNA samples isolated from the cell cultures and applying approaches of targeted molecular analysis to uncover potential pathway effects through gene expression profiling. Furthermore, the formulations investigated in this work contrast the addition of natural extract with chemical forms and evaluate…
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Taxonomy
TopicsDrug-Induced Hepatotoxicity and Protection · Phytochemicals and Antioxidant Activities · Genomics, phytochemicals, and oxidative stress