# Hepatotoxicity assessment of innovative nutritional supplements based on olive-oil formulations enriched with natural antioxidants

## TL;DR

This study evaluates the safety and benefits of olive-oil supplements with natural antioxidants for reducing oxidative stress and inflammation in diseases like Alzheimer's.

## Contribution

The study introduces innovative olive-oil formulations and evaluates their hepatotoxicity and molecular effects using targeted molecular analysis.

## Key findings

- Minimal cellular toxicity was observed in HepG2 cells exposed to the formulations.
- The supplements showed a significant positive impact on metabolic pathways related to oxidative stress.
- Natural antioxidant delivery modes were contrasted with chemical forms to assess cell toxicity.

## Abstract

This study focuses on the assessment of extra virgin olive-oil and olive fruit-based formulations enriched with natural antioxidants as potential nutritional supplements for alleviating symptoms and long-term consequences of illnesses whose molecular pathophysiology is affected by oxidative stress and inflammation, such as Alzheimer’s disease (AD).

Besides evaluating cell viability and proliferation capacity of human hepatocellular carcinoma HepG2 cells exposed to formulations in culture, hepatotoxicity was also considered as an additional safety measure using quantitative real-time PCR on RNA samples isolated from the cell cultures and applying approaches of targeted molecular analysis to uncover potential pathway effects through gene expression profiling. Furthermore, the formulations investigated in this work contrast the addition of natural extract with chemical forms and evaluate the antioxidant delivery mode on cell toxicity.

The results indicate minimal cellular toxicity and a significant beneficial impact on metabolic molecular pathways in HepG2 cell cultures, thus paving the way for innovative therapeutic strategies using olive-oil and antioxidants in dietary supplements to minimize the long-term effects of oxidative stress and inflammatory signals in individuals being suffered by disorders like AD.

Overall, the experimental design and the data obtained support the notion of applying innovative molecular methodologies and research techniques to evidently advance the delivery, as well as the scientific impact and validation of nutritional supplements and dietary products to improve public health and healthcare outcomes.