Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction

TL;DR

This paper presents a microfluidic platform that enables efficient crystallization and handling of pharmaceutical proteins for X-ray diffraction, allowing in situ and ex situ data collection to improve crystal quality and analysis.

Contribution

The study introduces a novel microfluidic approach for protein crystallization that simplifies crystal handling and enhances diffraction data collection.

Findings

Optimized crystallization conditions for a pharmaceutical protein.

Achieved high-quality diffraction data both in situ and ex situ.

Demonstrated the effectiveness of microfluidics in protein crystallography.

Abstract

We used a microfluidic platform to address the problems of obtaining diffraction quality crystals and crystal handling during transfer to the X-ray diffractometer. We optimize crystallization conditions of a pharmaceutical protein and collect X-ray data both in situ and ex situ.