Conjugate adaptive optics with remote focusing for three-dimensional focusing through scattering media

TL;DR

This paper introduces a novel conjugate adaptive optics with remote focusing system that significantly expands the correction volume for 3D imaging through scattering media, enabling faster and wider field of view in biological imaging.

Contribution

It presents a new CAORF system that maintains conjugation during 3D scanning, greatly increasing correction volume and imaging capabilities compared to traditional methods.

Findings

Achieved 10 times wider axial field of view.

Enabled 3D fluorescence imaging through scattering media.

Demonstrated multi-spot patterning through scattering layers.

Abstract

The small correction volume for conventional wavefront shaping methods limits their applications in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single correction by conjugate adaptive optics and remote focusing (CAORF). The remote focusing module can keep the conjugation between the AO and scattering layer during three-dimensional scanning. This new configuration provides a wider correction volume by the best utilization of the memory effect in a fast three-dimensional laser scanning microscope. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We also demonstrated three-dimensional fluorescence imaging and multi-spot patterning through a scattering layer.