# Conjugate adaptive optics with remote focusing for three-dimensional focusing through scattering media

## TL;DR

This paper introduces a novel conjugate adaptive optics with remote focusing system that significantly expands the correction volume for 3D imaging through scattering media, enabling faster and wider field of view in biological imaging.

## Contribution

It presents a new CAORF system that maintains conjugation during 3D scanning, greatly increasing correction volume and imaging capabilities compared to traditional methods.

## Key findings

- Achieved 10 times wider axial field of view.
- Enabled 3D fluorescence imaging through scattering media.
- Demonstrated multi-spot patterning through scattering layers.

## Abstract

The small correction volume for conventional wavefront shaping methods limits their applications in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single correction by conjugate adaptive optics and remote focusing (CAORF). The remote focusing module can keep the conjugation between the AO and scattering layer during three-dimensional scanning. This new configuration provides a wider correction volume by the best utilization of the memory effect in a fast three-dimensional laser scanning microscope. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We also demonstrated three-dimensional fluorescence imaging and multi-spot patterning through a scattering layer.

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Source: https://tomesphere.com/paper/1702.05163