Directionality of glioblastoma invasion in a 3d in vitro experiment

TL;DR

This study investigates the invasion patterns of glioblastoma cells in a 3D collagen gel, revealing a transient radial bias influenced by chemotactic factors and contact guidance, advancing understanding of tumor invasion mechanisms.

Contribution

It introduces a 3D in vitro model tracking individual glioblastoma cell paths and models their movement as radially biased, persistent random walks, highlighting dynamic invasion behavior.

Findings

Radial velocity bias was 20 microns/hr on day one.

Bias decayed significantly by day two.

Chemotactic factors and contact guidance influence invasion.

Abstract

Glioblastoma is the most malignant form of brain cancer. It is extremely invasive; the mechanisms that govern invasion are not well understood. To better understand the process of invasion, we conducted an in vitro experiment in which a 3d tumour spheroid is implanted into a collagen gel. The paths of individual invasive cells were tracked. These cells were modeled as radially biased, persistent random walkers. The radial velocity bias was found to be 20 microns/hr on day one, but decayed significantly by day two. The cause of this bias is thought to be due to chemotactic factors and contact guidance along collagen fibers.