A Self-Assembled Microlensing Rotational Probe
James P. Brody, Stephen R. Quake

TL;DR
This paper introduces a novel optical method using a self-assembled microsphere system to measure microscopic rotational motion, demonstrating its effectiveness in varying viscosities and potential for biological applications.
Contribution
The paper presents a new self-assembled microsphere technique for detecting microscopic rotational motion through optical signals influenced by azimuthal angles.
Findings
Successfully measured rotational diffusion constants in different viscosities.
Demonstrated the optical signal's dependence on azimuthal angle.
Discussed potential applications in biological systems.
Abstract
A technique to measure microscopic rotational motion is presented. When a small fluorescent polystyrene microsphere is attached to a larger polystyrene microsphere, the larger sphere acts as a lens for the smaller microsphere and provides an optical signal that is a strong function of the azimuthal angle. We demonstrate the technique by measuring the rotational diffusion constant of the microsphere in solutions of varying viscosity and discuss the feasibility of using this probe to measure rotational motion of biological systems.
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