Dynamically-Stabilized Pores in Bilayer Membranes

TL;DR

This paper introduces a feedback mechanism that dynamically stabilizes large pores in lipid bilayer membranes, explains the sudden closure phenomenon, and provides a method to measure pore line tension accurately.

Contribution

It presents a new feedback model for pore stabilization, explains the pore closure as a bifurcation, and offers a way to measure line tension from experimental data.

Findings

Pores can be stabilized dynamically via feedback.

Sudden pore closure is explained as a tangent bifurcation.

Line tension measured as 2.6 x 10^{-6} erg/cm for specific membrane mixture.

Abstract

Zhelev and Needham have recently created large, quasi-stable pores in artificial lipid bilayer vesicles [Biochim. Biophys. Acta 1147 (1993) 89]. Initially created by electroporation, the pores remain open for up to several seconds before quickly snapping shut. This result is surprising in light of the large line tension for holes in bilayer membranes and the rapid time scale for closure of large pores. We show how pores can be dynamically stabilized via a new feedback mechanism. We also explain quantitatively the observed sudden pore closure as a tangent bifurcation. Finally we show how Zhelev and Needham's experiment can be used to measure accurately the pore line tension, an important material parameter. For their SOPC/CHOL mixture we obtain a line tension of 2.6 10^{-6} erg/cm.