Observation of Fragile-to-Strong Dynamic Crossover in Protein Hydration Water

TL;DR

This study demonstrates that hydration water around lysozyme undergoes a fragile-to-strong dynamic crossover at 220 K, linking water's structural transition to protein flexibility and biological activity.

Contribution

It provides experimental evidence of a fragile-to-strong dynamic crossover in hydration water at 220 K, connecting water's structural transition to protein dynamics.

Findings

Hydration water exhibits a fragile-to-strong crossover at 220 K.

The transition involves a change from high-density to low-density water structures.

This crossover correlates with the onset of protein flexibility and function.

Abstract

At low temperatures proteins exist in a glassy state, a state which has no conformational flexibility and shows no biological functions. In a hydrated protein, at and above 220 K, this flexibility is restored and the protein is able to sample more conformational sub-states, thus becomes biologically functional. This 'dynamical' transition of protein is believed to be triggered by its strong coupling with the hydration water, which also shows a similar dynamic transition. Here we demonstrate experimentally that this sudden switch in dynamic behavior of the hydration water on lysozyme occurs precisely at 220 K and can be described as a Fragile-to-Strong dynamic crossover (FSC). At FSC, the structure of hydration water makes a transition from predominantly high-density (more fluid state) to low-density (less fluid state) forms derived from existence of the second critical point at an elevated pressure.