Enhancing protein drop stability for crystallization by chemical patterning

TL;DR

This paper introduces a chemical patterning technique to improve the stability of protein drops in crystallization processes, enhancing reproducibility and success rates.

Contribution

It demonstrates a scalable method of chemical patterning that significantly increases drop stability during handling and orientation changes.

Findings

Hydrophilic patterning increases drop stability.

Patterned slides improve reproducibility in crystallization.

Method is scalable and easy to implement.

Abstract

Motion of protein drops on crystallization media during routine handling is a major factor affecting the reproducibility of crystallization conditions. Drop stability can be enhanced by chemical patterning to more effectively pin the drop's contact line. As an example, a hydrophilic area is patterned on an initially flat hydrophobic glass slide. The drop remains confined to the hydrophilic area, and the maximum drop size that remains stable when the slide is rotated to the vertical position increases. This simple method is readily scalable and has the potential to significantly improve outcomes in hanging and sitting drop crystallization.