The cytoplasm of living cells: A functional mixture of thousands of components

TL;DR

This paper inventories the complex mixture of thousands of components in living cell cytoplasm, analyzing diffusion slowdown and phase separation, revealing insights into cellular biochemistry and physical properties.

Contribution

It provides a comprehensive inventory of cytoplasmic components and examines physical phenomena like diffusion and phase separation in this complex environment.

Findings

Diffusion in cytoplasm is significantly slower than in dilute solutions.

Phase separation is generally avoided despite high protein density.

Monomer-dimer equilibria are affected by crowding, slowing down by about twenty times.

Abstract

Inside every living cell is the cytoplasm: a fluid mixture of thousands of different macromolecules, predominantly proteins. This mixture is where most of the biochemistry occurs that enables living cells to function, and it is perhaps the most complex liquid on earth. Here we take an inventory of what is actually in this mixture. Recent genome-sequencing work has given us for the first time at least some information on all of these thousands of components. Having done so we consider two physical phenomena in the cytoplasm: diffusion and possible phase separation. Diffusion is slower in the highly crowded cytoplasm than in dilute solution. Reasonable estimates of this slowdown can be obtained and their consequences explored, for example, monomer-dimer equilibria are established approximately twenty times slower than in a dilute solution. Phase separation in all except exceptional cells appears not to be a problem, despite the high density and so strong protein-protein interactions present. We suggest that this may be partially a byproduct of the evolution of other properties, and partially a result of the huge number of components present.