Photon statistics and dynamics of Fluorescence Resonance Energy Transfer
Andrew J. Berglund, Andrew C. Doherty, Hideo Mabuchi
TL;DR
This paper presents high-resolution photon statistics measurements of FRET pairs, revealing photon antibunching and bunching phenomena, and suggests FRET photon statistics as a novel tool for studying DNA mechanics.
Contribution
It introduces high time-resolution photon statistics measurements of FRET, showing photon antibunching and bunching, and proposes using these statistics to study DNA mechanics.
Findings
Observation of photon antibunching and bunching in FRET pairs
Numerical simulation explains deviations from Forster theory
Potential application of FRET photon statistics in DNA mechanics studies
Abstract
We report high time-resolution measurements of photon statistics from pairs of dye molecules coupled by fluorescence resonance energy transfer (FRET). In addition to quantum-optical photon antibunching, we observe photon bunching on a timescale of several nanoseconds. We show by numerical simulation that configuration fluctuations in the coupled fluorophore system could account for minor deviations of our data from predictions of basic Forster theory. With further characterization we believe that FRET photon statistics could provide a unique tool for studying DNA mechanics on timescales from 10^-9 to 10^-3 s.
Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.