# Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes

## TL;DR

Researchers created brain organoids from single neural rosettes to study human brain development and how a genetic mutation in SHANK3 affects neurons and synapses.

## Contribution

The study introduces a novel organoid model from single neural rosettes to investigate human telencephalic development and SHANK3-related autism deficits.

## Key findings

- SNR-derived organoids contain diverse neural and glial cell types with organized structure.
- SHANK3 hemizygosity in organoids causes synaptic and intrinsic neuronal deficits and reduced clustered protocadherin expression.

## Abstract

Human telencephalon is an evolutionarily advanced brain structure associated with many uniquely human behaviors and disorders. However, cell lineages and molecular pathways implicated in human telencephalic development remain largely unknown. We produce human telencephalic organoids from stem cell-derived single neural rosettes and investigate telencephalic development under normal and pathological conditions. We show that single neural rosette-derived organoids contain pallial and subpallial neural progenitors, excitatory and inhibitory neurons, as well as macroglial and periendothelial cells, and exhibit predictable organization and cytoarchitecture. We comprehensively characterize the properties of neurons in SNR-derived organoids and identify transcriptional programs associated with the specification of excitatory and inhibitory neural lineages from a common pool of NPs early in telencephalic development. We also demonstrate that neurons in organoids with a hemizygous deletion of an autism- and intellectual disability-associated gene SHANK3 exhibit intrinsic and excitatory synaptic deficits and impaired expression of several clustered protocadherins. Collectively, this study validates SNR-derived organoids as a reliable model for studying human telencephalic cortico-striatal development and identifies intrinsic, synaptic, and clustered protocadherin expression deficits in human telencephalic tissue with SHANK3 hemizygosity.

Our understanding of human brain development in health and disease is limited. The authors generated human brain organoids from stem cell-derived isolated single neural rosettes to study human cortico-striatal development and deficits caused by an autism-associated genetic abnormality in SHANK3.