# Innate immune responses against the fungal pathogen Candida auris **Authors:** Yuanyuan Wang, Yun Zou, Xiaoqing Chen, Hao Li, Zhe Yin, Baocai Zhang, Yongbin Xu, Yiquan Zhang, Rulin Zhang, Xinhua Huang, Wenhui Yang, Chaoyue Xu, Tong Jiang, Qinyu Tang, Zili Zhou, Ying Ji, Yingqi Liu, Lingfei Hu, Jia Zhou, Yao Zhou, Jingjun Zhao, Ningning Liu, Guanghua Huang, Haishuang Chang, Wenxia Fang, Changbin Chen, Dongsheng Zhou PMC · DOI: 10.1038/s41467-022-31201-x · Nature Communications · 2022-06-21 ## TL;DR The paper compares immune responses to Candida auris and Candida albicans, finding that C. auris triggers a weaker immune reaction linked to its cell wall structure. ## Contribution The study reveals novel insights into how C. auris evades the immune system compared to C. albicans. ## Key findings - C. auris BJCA001 induces less immunoinflammation than C. albicans SC5314. - The differential immune response correlates with structural differences in the cell wall. - Findings suggest C. auris may evade immune detection more effectively. ## Abstract Candida auris is a multidrug-resistant human fungal pathogen responsible for nosocomial outbreaks worldwide. Although considerable progress has increased our understanding of the biological and clinical aspects of C. auris, its interaction with the host immune system is only now beginning to be investigated in-depth. Here, we compare the innate immune responses induced by C. auris BJCA001 and Candida albicans SC5314 in vitro and in vivo. Our results indicate that C. auris BJCA001 appears to be less immunoinflammatory than C. albicans SC5314, and this differential response correlates with structural features of the cell wall. Candida auris is a multidrug-resistant human fungal pathogen responsible for nosocomial outbreaks worldwide. Here, the authors identify differential innate immune responses induced by C. auris and Candida albicans in vitro and in vivo, which correlate with structural features of the cell wall. ## Linked entities - **Species:** Candida albicans (taxon 5476) ## Full-text entities - **Genes:** OCH1 (initiation-specific alpha-1,6-mannosyltransferase) [NCBI Gene 852845] {aka LDB12, NGD29}, Cxcl2 (C-X-C motif chemokine ligand 2) [NCBI Gene 20310] {aka CINC-2a, GROb, Gro2, MIP-2, MIP-2a, Mgsa-b}, CSF2 (colony stimulating factor 2) [NCBI Gene 1437] {aka CSF, GMCSF}, CLEC6A (C-type lectin domain containing 6A) [NCBI Gene 93978] {aka CLEC4N, CLECSF10, dectin-2, hDECTIN-2}, PMR1 (Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1) [NCBI Gene 852709] {aka BSD1, LDB1, SSC1}, ATP2C1 (ATPase secretory pathway Ca2+ transporting 1) [NCBI Gene 27032] {aka ATP2C1A, BCPM, HHD, PMR1, SPCA1, hSPCA1}, PMT1 (dolichyl-phosphate-mannose-protein mannosyltransferase PMT1) [NCBI Gene 851462], Adgre1 (adhesion G protein-coupled receptor E1) [NCBI Gene 13733] {aka DD7A5-7, EGF-TM7, Emr1, F4/80, Gpf480, Ly71}, Fcgr2b (Fc receptor, IgG, low affinity IIb) [NCBI Gene 14130] {aka CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a}, Fcgr3 (Fc receptor, IgG, low affinity III) [NCBI Gene 14131] {aka CD16}, MROS (Melkersson-Rosenthal syndrome) [NCBI Gene 8011] {aka MRS}, CXCL1 (C-X-C motif chemokine ligand 1) [NCBI Gene 2919] {aka FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3}, TNF (tumor necrosis factor) [NCBI Gene 7124] {aka DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F}, mannosyltransferase [NCBI Gene 28875843], CXCL2 (C-X-C motif chemokine ligand 2) [NCBI Gene 2920] {aka CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, MIP2}, MAPK8 (mitogen-activated protein kinase 8) [NCBI Gene 5599] {aka JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8}, CD209 (CD209 molecule) [NCBI Gene 30835] {aka CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, hDC-SIGN}, Dnase1 (deoxyribonuclease I) [NCBI Gene 13419] {aka DNaseI, Dnl1}, Itgam (integrin alpha M) [NCBI Gene 16409] {aka CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40} - **Diseases:** inflammation (MESH:D007249), acid-labile mannan (MESH:D005166), Fungal (MESH:D009181), lethargy (MESH:D053609), infected (MESH:D007239), invasive fungal infections (MESH:D000072742), bloodstream infection (MESH:D018805), C. auris infection (MESH:C000656864), abscess (MESH:D000038), adenocarcinoma cancer (MESH:D009369), neutropenic (MESH:D044504), systemic Candidiasis (MESH:C536777), colorectal adenocarcinoma (MESH:D003110), inflammatory cytokines (MESH:D000080424), invasive candidiasis (MESH:D058365), weight loss (MESH:D015431), cytotoxicity (MESH:D064420), (MESH:D002177) - **Chemicals:** monosaccharide (MESH:D009005), HCl (MESH:D006851), sugar (MESH:D000073893), streptomycin (MESH:D013307), PS (MESH:D010758), beta-(1,3)-glucan (MESH:C033363), phenol red (MESH:D010637), FITC (MESH:D016650), Alcian Blue (MESH:D000423), phospholipomannan (MESH:C400188), Congo Red (MESH:D003224), Calcofluor White (MESH:C007061), SYTOX Green (MESH:C402795), pyruvate (MESH:D019289), CO2 (MESH:D002245), hematoxylin (MESH:D006416), DAPI (MESH:C007293), agar (MESH:D000362), penicillin (MESH:D010406), glucan (MESH:D005936), alkali (MESH:D000468), paraffin (MESH:D010232), polysaccharide (MESH:D011134), glycosylphosphatidylinositol (MESH:D017261), Mannan (MESH:D008351), glycerol (MESH:D005990), osmium tetroxide (MESH:D009993), Periodic acid (MESH:D010504), nitrogen (MESH:D009584), Percoll (MESH:C016039), formaldehyde (MESH:D005557), phosphomannan (MESH:C005437), carbohydrate (MESH:D002241), PI (MESH:D010716), Dulbecco's modified Eagle's medium (-), water (MESH:D014867), 2-mercaptoethanol (MESH:D008623), NaCl (MESH:D012965), H&E. (MESH:D006371), beta-(1,6)-glucan (MESH:C064197), PBS (MESH:D007854), uranyl acetate (MESH:C005460), polystyrene (MESH:D011137), Fluconazole (MESH:D015725), AlexaFluor 488 (MESH:C000711379), acetone (MESH:D000096), tetracycline (MESH:D013752), Triton X-100 (MESH:D017830), chitin (MESH:D002686), dextrose (MESH:D005947), paraformaldehyde (MESH:C003043), L-glutamine (MESH:D005973), resin (MESH:D012116), EDTA (MESH:D004492), beta-glucan (MESH:D047071), doxycycline (MESH:D004318), SDS (MESH:D012967), methanol (MESH:D000432), copper (MESH:D003300), PVDF (MESH:C024865) - **Species:** Nakaseomyces glabratus (species) [taxon 5478], Galleria mellonella (greater wax moth, species) [taxon 7137], Mus musculus (house mouse, species) [taxon 10090], Candida albicans (species) [taxon 5476], Danio rerio (leopard danio, species) [taxon 7955], Homo sapiens (human, species) [taxon 9606], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Candida [taxon 1535326], Candida albicans SC5314 (strain) [taxon 237561], Candidozyma auris (species) [taxon 498019] - **Mutations:** C in 3, L120C, T9300A - **Cell lines:** SC5314 — Homo sapiens (Human), Embryonic stem cell (CVCL_6F20), HaCat — Homo sapiens (Human), Spontaneously immortalized cell line (CVCL_0038), C57BL/6 — Mus musculus (Mouse), Transformed cell line (CVCL_C0MU), A549 — Homo sapiens (Human), Lung adenocarcinoma, Cancer cell line (CVCL_0023), HeLa — Homo sapiens (Human), Human papillomavirus-related endocervical adenocarcinoma, Cancer cell line (CVCL_0030), L929 — Mus musculus (Mouse), Spontaneously immortalized cell line (CVCL_AR58), HUVEC — Homo sapiens (Human), Finite cell line (CVCL_3722), Caco-2 — Homo sapiens (Human), Colon adenocarcinoma, Cancer cell line (CVCL_0025) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC9213489/full.md ## Figures 7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC9213489/full.md ## References 69 references — full list in the complete paper: https://tomesphere.com/paper/PMC9213489/full.md --- Source: https://tomesphere.com/paper/PMC9213489