Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity
Junyan Ma, Liqi Zhang, Hong Zhan, Yun Mo, Zuanjie Ren, Anwen Shao, Jun Lin
TL;DR
This study uses single-cell analysis to explore endometriosis, revealing fibroblast roles and immune cell changes that may explain the disease's development.
Contribution
The study provides a single-cell transcriptome atlas of endometriosis, identifying fibroblast subpopulations and immune cell heterogeneity linked to the disease.
Findings
Fibroblast subpopulations and a developmental trajectory were identified in endometriosis.
T cells in endometriosis were less activated, while monocytes/macrophages increased in lesions.
Cell-cell interactions showed an imbalanced immune environment promoting endometriosis.
Abstract
Endometriosis is an oestrogen-dependent disease with an unclear aetiology and pathogenesis affecting 6–10% of the global female population, predominantly those of reproductive age. Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectopic endometrium, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women to create a single-cell transcriptome atlas of endometriosis. We have identified 9 cell types and performed single-cell analysis of fibroblasts, and determined a potential developmental trajectory associated with endometriosis. We also identified fibroblast subpopulations related to endometriosis development and found that StAR played an important role in this process. Moreover, T cells in endometriosis were less activated or inflammatory with decreased effector CD8 + T cells, while the…
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Taxonomy
TopicsEndometriosis Research and Treatment · Reproductive System and Pregnancy · Endometrial and Cervical Cancer Treatments