# Study of microbiome changes in patients with ulcerative colitis in the Central European part of Russia

## TL;DR

This study examines how the gut microbiome differs in patients with ulcerative colitis in Central European Russia compared to healthy individuals.

## Contribution

The study identifies novel bacterial taxa in ulcerative colitis patients and provides insights into microbiome changes specific to the Russian population.

## Key findings

- Patients with ulcerative colitis showed increased abundance of genera like Haemophilus and Prevotella.
- A significant decrease in genera such as Fusicatenibacter and Lactococcus was observed in ulcerative colitis patients.
- New taxa like Negativibacillus spp. and Falsochrobactrum spp. were identified in human stool samples for the first time.

## Abstract

Ulcerative colitis (UC) is an inflammatory disease that affects the colon and rectum. Recently, evidence has emerged about the influence of microbiota on the development of this disease. However, studies on the role of intestinal microbiota in the pathogenesis of UC have been incomplete. In addition, there are no comprehensive studies of the causes of ulcerative colitis and data on the microbiological composition of the intestines of patients with ulcerative colitis in Russia. We carried out a study of the microbiological composition of the intestines of patients with ulcerative colitis and healthy individuals. We found significant changes in the bacteria genera and species in patients with UC compared with the control group using sequencing on the IonTorrent PGM system and subsequent data analysis. In our study we observed a significant increase of the genus Haemophilus, Olsenella, Prevotella, Cedecea, Peptostreptococcus, Faecalibacterium, Lachnospira, Negativibacillus, Butyrivibrio, and the species Bacteroides coprocola, Phascolarctobacterium succinatutens, Dialister succinatiphilus, Sutterella wadsworthensis, Faecalibacterium prausnitzii in patients with ulcerative colitis. In addition, in patients with ulcerative colitis there was a significant decrease in the genus Fusicatenibacter, Butyricimonas, Lactococcus, Eisenbergiella, Coprobacter, Cutibacterium, Falsochrobactrum, Brevundimonas, Yersinia, Leuconostoc and in the species Fusicatenibacter saccharivorans. We found confirmation of our data with literary sources and studies of UC. In addition, we discovered a few taxa such as Negativibacillus spp. and Falsochrobactrum spp. that have not been previously found in human stool samples. Our data confirm that more research is needed to understand the role of microbiome changes in the development of UC in different people populations.

Intestinal microbiota, ulcerative colitis, inflammatory bowel disease, sequencing, Russian population.