# The Novel Functions of the PLC/PKC/PKD Signaling Axis in G Protein-Coupled Receptor-Mediated Chemotaxis of Neutrophils **Authors:** Xuehua Xu, Tian Jin PMC · DOI: 10.1155/2015/817604 · Journal of Immunology Research · 2015-10-29 ## TL;DR This paper explores how the PLC/PKC/PKD signaling pathway helps neutrophils move toward inflammation sites by responding to chemical signals. ## Contribution The study reveals new roles of the PLC/PKC/PKD signaling axis in neutrophil chemotaxis. ## Key findings - The PLC/PKC/PKD axis is crucial for GPCR-mediated chemotaxis in neutrophils. - Activation of this signaling pathway controls directional cell migration in response to chemoattractants. ## Abstract Chemotaxis, a directional cell migration guided by extracellular chemoattractant gradients, plays an essential role in the recruitment of neutrophils to sites of inflammation. Chemotaxis is mediated by the G protein-coupled receptor (GPCR) signaling pathway. Extracellular stimuli trigger activation of the PLC/PKC/PKD signaling axis, which controls several signaling pathways. Here, we concentrate on the novel functions of PLC/PKC/PKD signaling in GPCR-mediated chemotaxis of neutrophils. ## Linked entities - **Proteins:** HSPG2 (heparan sulfate proteoglycan 2), PRRT2 (proline rich transmembrane protein 2), PRKD1 (protein kinase D1) ## Full-text entities - **Genes:** DSTN (destrin, actin depolymerizing factor) [NCBI Gene 11034] {aka ACTDP, ADF, HEL32, bA462D18.2}, PRKCA (protein kinase C alpha) [NCBI Gene 5578] {aka AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha}, VEGFA (vascular endothelial growth factor A) [NCBI Gene 7422] {aka L-VEGF, MVCD1, VEGF, VPF}, RAC2 (Rac family small GTPase 2) [NCBI Gene 5880] {aka EN-7, Gx, HSPC022, IMD73A, IMD73B, IMD73C}, PTAFR (platelet activating factor receptor) [NCBI Gene 5724] {aka PAFR}, PLCB2 (phospholipase C beta 2) [NCBI Gene 5330] {aka PLC-beta-2}, PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) [NCBI Gene 5294] {aka IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma}, CFL2 (cofilin 2) [NCBI Gene 1073] {aka NEM7}, AZU1 (azurocidin 1) [NCBI Gene 566] {aka AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC}, PRKCD (protein kinase C delta) [NCBI Gene 5580] {aka ALPS3, CVID9, MAY1, PKCD, nPKC-delta}, Rac2 (Rac family small GTPase 2) [NCBI Gene 19354], SSH2 (slingshot protein phosphatase 2) [NCBI Gene 85464] {aka SSH-2, SSH-2L}, CDC42 (cell division cycle 42) [NCBI Gene 998] {aka CDC42Hs, G25K, TKS}, SUCLG1 (succinate-CoA ligase GDP/ADP-forming subunit alpha) [NCBI Gene 8802] {aka GALPHA, MTDPS9, SUCLA1}, Plcg1 (phospholipase C, gamma 1) [NCBI Gene 18803] {aka Cded, Plc-1, Plc-gamma1, Plcg-1}, NCF1 (neutrophil cytosolic factor 1) [NCBI Gene 653361] {aka CGD1, NCF-1, NCF-47K, NCF1A, NOXO2, SH3PXD1A}, MYH14 (myosin heavy chain 14) [NCBI Gene 79784] {aka DFNA4, DFNA4A, FP17425, MHC16, MYH17, NMHC II-C}, Pten (phosphatase and tensin homolog) [NCBI Gene 19211] {aka 2310035O07Rik, A130070J02Rik, B430203M17Rik, MMAC1, PTENbeta, TEP1}, PLCB3 (phospholipase C beta 3) [NCBI Gene 5331] {aka SMDCD}, Lrpap1 (low density lipoprotein receptor-related protein associated protein 1) [NCBI Gene 16976] {aka HBP44, RAP}, Rhoa (ras homolog family member A) [NCBI Gene 11848] {aka Arha, Arha1, Arha2}, RAC1 (Rac family small GTPase 1) [NCBI Gene 5879] {aka MIG5, MRD48, Rac-1, TC-25, p21-Rac1}, PRKCB (protein kinase C beta) [NCBI Gene 5579] {aka PKC-beta, PKCB, PKCI(2), PKCbeta, PRKCB1, PRKCB2}, Gsk3b (glycogen synthase kinase 3 beta) [NCBI Gene 56637] {aka 7330414F15Rik, 8430431H08Rik, GSK-3, GSK-3beta, GSK3}, HSPG2 (heparan sulfate proteoglycan 2) [NCBI Gene 3339] {aka HSPG, PLC, PRCAN, SJA, SJS, SJS1}, PTEN (phosphatase and tensin homolog) [NCBI Gene 5728] {aka 10q23del, BZS, CWS1, DEC, GLM2, MHAM}, GNA12 (G protein subunit alpha 12) [NCBI Gene 2768] {aka HG1M1, NNX3, RMP, gep}, PLCG2 (phospholipase C gamma 2) [NCBI Gene 5336] {aka APLAID, FCAS3, PLC-IV, PLC-gamma-2}, Pik3cg (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) [NCBI Gene 30955] {aka 5830428L06Rik, PI3Kgamma, p110gamma, p120-PI3K}, C5AR1 (complement C5a receptor 1) [NCBI Gene 728] {aka C5A, C5AR, C5R1, CD88}, RHO (rhodopsin) [NCBI Gene 6010] {aka CSNBAD1, OPN2, RP4}, PRRT2 (proline rich transmembrane protein 2) [NCBI Gene 112476] {aka BFIC2, BFIS2, DSPB3, DYT10, EKD1, FICCA}, EGF (epidermal growth factor) [NCBI Gene 1950] {aka HOMG4, URG}, CFL1 (cofilin 1) [NCBI Gene 1072] {aka CFL, HEL-S-15, cofilin}, Plce1 (phospholipase C, epsilon 1) [NCBI Gene 74055] {aka 4933403A21Rik, PLCepsilon, Plce, mKIAA1516}, Plcg2 (phospholipase C, gamma 2) [NCBI Gene 234779] {aka PLC-gamma-2, PLCgamma2, Plcg-2}, CXCL8 (C-X-C motif chemokine ligand 8) [NCBI Gene 3576] {aka GCP-1, GCP1, IL8, LECT, LUCT, LYNAP}, IL1B (interleukin 1 beta) [NCBI Gene 3553] {aka IL-1, IL1-BETA, IL1F2, IL1beta}, PDXP (pyridoxal phosphatase) [NCBI Gene 57026] {aka CIN, PLP, dJ37E16.5}, Plcb2 (phospholipase C, beta 2) [NCBI Gene 18796] {aka B230205M18Rik, B230399N12}, PKD2 (polycystin 2, transient receptor potential cation channel) [NCBI Gene 5311] {aka APKD2, PC2, PKD4, Pc-2, TRPP2}, PKD1 (polycystin 1, transient receptor potential channel interacting) [NCBI Gene 5310] {aka PBP, PC1, Pc-1, TRPP1, eliosin}, CXCR6 (C-X-C motif chemokine receptor 6) [NCBI Gene 10663] {aka BONZO, CD186, CDw186, STRL33, TYMSTR}, PRKD1 (protein kinase D1) [NCBI Gene 5587] {aka CHDED, PKC-MU, PKCM, PKD, PKD1, PRKCM}, PLCG1 (phospholipase C gamma 1) [NCBI Gene 5335] {aka IDAA, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1}, Gpbar1 (G protein-coupled bile acid receptor 1) [NCBI Gene 227289] {aka BG37, GPCR, GPR131, M-BAR, TGR5} - **Diseases:** bacterial and fungal infections (MESH:D009181), arthritis (MESH:D001168), inflammation (MESH:D007249), leukemia (MESH:D007938), pulmonary fibrosis (MESH:D011658), asthma (MESH:D001249), cancer (MESH:D009369) - **Chemicals:** calcium (MESH:D002118), GDP (MESH:D006153), GO6983 (MESH:C465664), DAG (MESH:D004075), Alexa 594 (MESH:C417664), phorbol ester (MESH:D010703), PIP2 (MESH:D019269), phospholipids (MESH:D010743), IP3 (MESH:D015544), fMLP (MESH:D009240), U73122 (MESH:C060229), Ca2+ (-), PtdIns(3,4,5)P3 (MESH:C060974), serine (MESH:D012694), superoxide (MESH:D013481), ATP (MESH:D000255) - **Species:** Mus musculus (house mouse, species) [taxon 10090], Dictyostelium discoideum (species) [taxon 44689], Homo sapiens (human, species) [taxon 9606] - **Mutations:** Ser/Thr, K612W - **Cell lines:** insect — Trichoplusia ni (Cabbage looper), Spontaneously immortalized cell line (CVCL_C190), HL60 — Homo sapiens (Human), Adult acute myeloid leukemia with maturation, Cancer cell line (CVCL_0002) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC4641950/full.md ## Figures 3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC4641950/full.md ## References 68 references — full list in the complete paper: https://tomesphere.com/paper/PMC4641950/full.md --- Source: https://tomesphere.com/paper/PMC4641950