Research on Acute Toxicity and Pharmacodynamics of Jinyulian Oral Solution

TL;DR

This study tested a herbal solution's safety and antibacterial effects in mice and found it safe with strong antibacterial properties.

Contribution

The study provides new evidence on the safety and antibacterial efficacy of Jinyulian Oral Solution in both in vitro and in vivo models.

Findings

Jinyulian Oral Solution showed no acute toxicity in mice even at 250 times the clinical dose.

The solution exhibited significant antibacterial effects against Staphylococcus aureus and Escherichia coli.

MIC50 and MIC90 values indicated effective inhibition of bacterial growth at measurable concentrations.

Abstract

A research on Jinyulian Oral Solution was conducted and the objectives were to discover its possible acute toxicity and antibacterial effects when used in vitro and in vivo. Regarding the acute toxicity test, Kunming mice were fed a maximum amount of the solution as their stomachs could hold, i.e., 40 mL kg−1. To ascertain the minimum inhibitory concentration (MIC) of the solution, two types of germs, i.e., Staphylococcus aureus and Escherichia coli, were selected and tube dilution method was adopted. An antibacterial experimental model relying on animals’ body was developed for the researchers to observe the solution’s antibacterial effects. Test results showed that no abnormalities were discovered within 14 days after the initial date of testing and the mice grew as normal when fed with an amount of the solution 250 times of a normal clinical doze (In this case a man was assumed to weigh 60 kg.) and that the solution demonstrated obvious antibacterial effects on the two types of selected germs. The respective measured MIC50 and MIC90 values of the two germs were 3.2, 12.8, 6.4, and 25.6 mg L−1. Therefore, it is reasonable to conclude that Jinyulian Oral Solution possesses no acute toxicity but obvious antibacterial effects on the two before-mentioned germs.