# Genome-wide identification of copy number variations between two chicken lines that differ in genetic resistance to Marek’s disease

## TL;DR

This study identifies genome-wide copy number variations in two chicken lines with different resistance to Marek’s disease, offering insights into genetic factors influencing disease susceptibility.

## Contribution

The study provides a comprehensive genome-wide identification of CNVs associated with Marek’s disease resistance in inbred chicken lines.

## Key findings

- A total of 5,680 CNV regions were identified, with 1,546 and 1,866 specific to the resistant and susceptible chicken lines, respectively.
- CNVRs in the susceptible line were enriched in genes related to the MAPK signaling pathway, suggesting its role in disease response.

## Abstract

Copy number variation (CNV) is a major source of genome polymorphism that directly contributes to phenotypic variation such as resistance to infectious diseases. Lines 63 and 72 are two highly inbred experimental chicken lines that differ greatly in susceptibility to Marek’s disease (MD), and have been used extensively in efforts to identify the genetic and molecular basis for genetic resistance to MD. Using next generation sequencing, we present a genome-wide assessment of CNVs that are potentially associated with genetic resistance to MD.

Three chickens randomly selected from each line were sequenced to an average depth of 20×. Two popular software, CNVnator and Pindel, were used to call genomic CNVs separately. The results were combined to obtain a union set of genomic CNVs in the two chicken lines.

A total of 5,680 CNV regions (CNVRs) were identified after merging the two datasets, of which 1,546 and 1,866 were specific to the MD resistant or susceptible line, respectively. Over half of the line-specific CNVRs were shared by 2 or more chickens, reflecting the reduced diversity in both inbred lines. The CNVRs fixed in the susceptible lines were significantly enriched in genes involved in MAPK signaling pathway. We also found 67 CNVRs overlapping with 62 genes previously shown to be strong candidates of the underlying genes responsible for the susceptibility to MD.

Our findings provide new insights into the genetic architecture of the two chicken lines and additional evidence that MAPK signaling pathway may play an important role in host response to MD virus infection. The rich source of line-specific CNVs is valuable for future disease-related association studies in the two chicken lines.

The online version of this article (doi:10.1186/s12864-015-2080-5) contains supplementary material, which is available to authorized users.