# MicroRNAs in urine are not biomarkers of multiple myeloma

**Authors:** Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková

PMC · DOI: 10.1186/s12952-015-0035-7 · 2015-09-23

## TL;DR

This study found that microRNAs in urine are not reliable biomarkers for multiple myeloma.

## Contribution

The study provides evidence against the use of urine microRNAs as biomarkers for multiple myeloma.

## Key findings

- Twenty microRNAs were found deregulated in urine samples, but most did not reach statistical significance.
- Only miR-22-3p showed near-significant differences in expression levels.
- The results do not support urine microRNAs as a viable biomarker for multiple myeloma.

## Abstract

In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.

Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.

Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

## Linked entities

- **Diseases:** multiple myeloma (MONDO:0009693)

## Full-text entities

- **Genes:** MIR17HG (miR-17-92a-1 cluster host gene) [NCBI Gene 407975] {aka C13orf25, LINC00048, MIHG1, MIRH1, MIRHG1, NCRNA00048}, MIR29B1 (microRNA 29b-1) [NCBI Gene 407024] {aka MIRN29B1, miR-29b, miRNA29B1, mir-29b-1}, MIR200C (microRNA 200c) [NCBI Gene 406985] {aka MIRN200C, mir-200c}, BCL2L11 (BCL2 like 11) [NCBI Gene 10018] {aka BAM, BIM, BOD}, MIR29C (microRNA 29c) [NCBI Gene 407026] {aka MIRN29C, miRNA29C, mir-29c}, MIR29A (microRNA 29a) [NCBI Gene 407021] {aka MIRN29, MIRN29A, hsa-mir-29, hsa-mir-29a, miRNA29A, mir-29a}, MIR106B (microRNA 106b) [NCBI Gene 406900] {aka MIRN106B, mir-106b}, MIR15A (microRNA 15a) [NCBI Gene 406948] {aka MIRN15A, hsa-mir-15a, miRNA15A, mir-15a}, MIR96 (microRNA 96) [NCBI Gene 407053] {aka DFNA50, MIRN96, hsa-mir-96, miR-96, miRNA96}, MIR183 (microRNA 183) [NCBI Gene 406959] {aka MIRN183, miR-183, miRNA183}, MIR223 (microRNA 223) [NCBI Gene 407008] {aka MIRN223, miRNA223, mir-223}, CEL (carboxyl ester lipase) [NCBI Gene 1056] {aka BAL, BSDL, BSSL, CELL, CEase, FAP}, MIR126 (microRNA 126) [NCBI Gene 406913] {aka MIRN126, miRNA126, mir-126}, MIR18A (microRNA 18a) [NCBI Gene 406953] {aka C13orf25, MIR18, MIRH1, MIRHG1, MIRN18, MIRN18A}, MIR22 (microRNA 22) [NCBI Gene 407004] {aka MIRN22, hsa-mir-22, miR-22}, MYC (MYC proto-oncogene, bHLH transcription factor) [NCBI Gene 4609] {aka MRTL, MYCC, bHLHe39, c-Myc}
- **Diseases:** DN (MESH:D003928), MGUS (MESH:D008998), HD (MESH:D000067329), MM (MESH:D009101), kidney damage (MESH:D007674), hematological malignancies (MESH:D019337), bladder cancer (MESH:D001749), UC (MESH:D014523), metastasis (MESH:D009362), renal insufficiency (MESH:D051437), urologic cancers (MESH:D014571), HD RCC MM (MESH:D002292), malignancy of plasma cells (MESH:D054219)
- **Chemicals:** EDTA (MESH:D004492),  (MESH:D014408)
- **Species:** Homo sapiens (human, species) [taxon 9606]
- **Cell lines:** VK — Homo sapiens (Human), Transformed cell line (CVCL_6471)

## Figures

2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC4581458/full.md

---
Source: https://tomesphere.com/paper/PMC4581458