# A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

## TL;DR

A new method using a special probe and salt-induced effects detects genetic mutations at room temperature, making it accessible for labs without advanced equipment.

## Contribution

A novel hybridization system using a locked nucleic acid probe and selective salt-induced effect for SNP detection at room temperature.

## Key findings

- The hybridization system works effectively at room temperature.
- The method successfully detected SNPs (MDR1 C3435T/A) from 45 volunteers.
- Results matched those from pyrophosphoric acid sequencing.

## Abstract

Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.