Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

TL;DR

This study uses proteomic analysis to explore how the Snail1 transcription factor affects the differentiation of mouse stem cells and fibroblasts.

Contribution

The study provides new proteomic data on Snail1's role in nuclear regulation during cell differentiation.

Findings

Snail1 expression disrupts osteoblast and adipocyte differentiation in murine mesenchymal stem cells.

Proteomic analysis focused on nuclear fractions to understand Snail1's regulatory effects on transcription factors.

Data from SILAC and TMT labeling methods were used to compare Snail1-expressing and control cells.

Abstract

The transcription factor (TF) Snail1 is a major inducer of the epithelial–mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

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