# Analytical sequence to study G-CSF effect on the transcriptome of isolated spinal motoneurons from SOD1 G93A mice, an animal model for amyotrophic lateral sclerosis

## TL;DR

This study examines how G-CSF affects gene activity in spinal motor neurons of mice with a model of amyotrophic lateral sclerosis.

## Contribution

The paper provides quality controls and details the experimental strategy for a microarray study on G-CSF's effects.

## Key findings

- G-CSF re-adjusted gene expression in motoneurons of symptomatic SOD1G93A mice.
- G-CSF modulates genes related to neuromuscular function in the studied animal model.

## Abstract

Granulocyte-colony stimulating factor (G-CSF) has been recently identified as a neurotrophic factor able to preserve motor functions, rescue motor units and extent survival in an animal model of amyotrophic lateral sclerosis, the SOD1 G93A mice. To gain insight into the mode of action of G-CSF, we have recently performed gene expression profiling on isolated lumbar motoneurons from SOD1G93A mice, and shown that G-CSF re-adjusted gene expression in motoneurons of symptomatic SOD1G93A mice and modulates genes related to neuromuscular function (Henriques et al., 2015). Here, we provide quality controls for the microarray experiment (GO accession number GSE60856) and describe the experimental strategy.